自由基/活性氧分析

衆多化學(xué)和生化反應過(g小看uò)程都(dōu)涉及自由基的參與，研究自由基的産生、結構及化學(x樂窗ué)反應性能(néng)至關重要，目前形成(chéng)了“自由基化學(xué)”。此外，現代生物學我歌(xué)認為細胞呼吸獲取氧份，多餘的氧會(huì)轉化匠快成(chéng)氧自由基。由于這(zhè)種(z短空hǒng)物質非常活躍，幾乎可以與各種(zhǒng)物質發(fā)的票生作用，引起(qǐ)一系列對(duì)車間細胞具有破壞性的連鎖反應，從而損壞人體正常細胞和組織，引起(店懂qǐ)多種(zhǒng)疾病，這(zhè)是人類衰老和患病的根源。另一方面聽路(miàn)，活性氧含量與生理過(guò)程密切相關，特别河一是許多病變過(guò)程都(dōu)伴随著(章如zhe)活性氧含量水平的明顯異常變化。因此活性氧研究成(chéng)為少議了生命科學(xué)的一研究熱點課題。

   自由基/活性氧具有很高的反應活性，發(fā)展靈敏、準确的自由基鄉風或活性氧檢測方法引起(qǐ)了廣大科研工作者的興趣。與其它自由基/活性氧測定方法相比，化學(xué)發(fā)光除具有條件溫和、儀拿討器簡單、操作方便、靈敏度高等優點外，還(hái)具火購有發(fā)光現象與化學(xué)反應同時(shí)發(fā)生的特點，大文因此將(jiāng)化學(xué)發(fā)光的瞬時(shí)性與自由基短壽中鄉命相結合具有很好(hǎo)的應用前景。BPCL超微弱發(fā)光測量儀以其優異的靈敏度和良好(hǎo)的穩定性，在自由基/活笑街性氧分析上具有重要應用，其例子有見唱：

[1]    &nbs農呢p;  Zhu B Z, Mao L, Huang C H, Fan 少還R M, Kalyanaraman B, Zhu J G. Unp弟信recedented hydroxyl radi森窗cal-dependent two-step chemilumine我信scence productio師區n by polyhalogenate也地d quinoid carcinogens a從西nd H₂O₂. Proceedings of the National Academ路店y of Sciences of the United States兵短 of America, 2012, 109(40): 16046-16051.

[2]    &nb要門sp;  Wang D B, Zhao L X, Guo L H, et al. 短樹Online detection 相車of reactive oxygen species 坐她in ultraviolet (UV)-irradiat錢這ed nano-TiO₂ suspensions by continuous fl美區ow chemiluminescence. Analytical Chemistry, 2014, 86(21): 105影熱35-10539.

[3]   放人    Zou F J, Zhou W J舞吃, Guan W J, Lu 看分C, Tang B Z. Screening of p雜到hotosensitizers by東討 chemiluminescen好農ce monitoring o錢河f formation dynamics o黑什f singlet oxygen during p話行hotodynamic therapy. Analytical Chemistry, 2016, 88(19): 9707-9713.

[4]     &nbs場上p; Zhu Y S, Li M, Wang 到照X H, Jin H J, Liu S S, Xu J X去器, Chen Q. Caspase cleavage of cytoch科內rome c1 disrupts mitochondrial通農 function and enhanc用生es cytochrome c release. Cell Research, 2012, 22(1): 12森拿7-141.

[5]     &n嗎國bsp; Zhang G M, J, Liu G. E都著ffect of dipheny雨些l dimethyl bicarboxylate o光多n concanavalin A-induced liver injur森票y in mice. Liver International, 2005, 25(4): 904-912.

[6]       Guo S H, Bezard E, Zhao B L. Prote區化ctive effect of green tea西樂 polyphenols on the SH-SY5Y西會 cells against 6-OHDA induce道懂d apoptosis through ROS-謝妹NO pathway. Free Radical Bio厭暗logy & Medicine, 2005, 39(5): 682-695.

[7]   吧朋;    Lin Y, Jia R, Liu Y, et al.輛視 Diosgenin inhibi雨子ts superoxide generation in F北車MLP-activated mou知場se neutrophils v為麗ia multiple pathways. Free Radical Research, 2014, 48(12): 1485-1493.

[8]    &n見弟bsp;  Quan Y, Jiang C T, Xue B, Zhu S G, Wang要南 X. High glucose stimulates 南西TNFα and MCP-1 expressi紅北on in rat microglia via ROS and NF-κB 站窗pathways. Acta Pharmacologica Sinica, 2011, 32(2): 188-193.

[9]   &nbs山舞p;   Chang L, Zhang Z M, Li W J, Dai J,員雨 Guan Y F, Wang X. Liver-X-recept暗離or activator preve女日nts homocysteine-induced pro房都duction of IgG antibodies from m河議urine B lymphocytes via the ROS-NF-κB 慢作pathway. Biochemical & Biophysical Re工睡search Communications, 2007, 357(3): 7謝舊72-778.

[10]    Chen A, Cao E T, Qin J F Qin J F可長. Arsenite-induced reacti農短ve oxygen species and the repression知海 of α-tocopherol in the MGC-大遠803 cells. European Journal of Pha器銀rmacology, 2002, 448(1): 11-18也內.

[11]    Zhou W J, Cao Y Q, Sui D D, Lu C. Rad妹舊ical pair-driven luminesce銀吧nce of quantum dots for spec事看ific detection of perox外火ynitrite in living cells. Analytical Chemistry, 2016,88(5): 2659-2665.

[12]    Lin J M, Liu M L. Sin但工glet oxygen generated from冷劇 the decomposition of peroxymonocarbo做媽nate and its observation with chemilumi草森nescence method. Spectrochimica Acta Par家什t A Molecular & Bio訊刀molecular Spectroscopy, 2009,72(1):126-132.